吴忠义课题组

来源:农业生物技术研究中心

课题组介绍

根系是植物的生存之本,是植物吸取水分和营养元素的关键器官。拥有一个强壮的根系对植物的营养生长和生殖生长非常重要,但是以玉米根系结构(RSA)作为性状来提高玉米产量和增强抗逆性方面的研究进展却很缓慢。玉米分子育种课题组主要研究内容集中在以下几个方面:一是利用300多份玉米自然变异群体材料,解析玉米根系生长发育的分子调控机制,解析有重要应用价值的代谢途径,挖掘主效调控基因,通过促进根系生长创制抗逆玉米新种质材料;二是建立不受基因型限制的玉米骨干自交系的高效规模化DNA导入体系;三是建立玉米骨干自交系的基因组编辑技术体系,利用编辑技术在商品化自交系中创制常规技术难以实现的玉米新种质材料。课题组组建近5年来,连续两次承担国家转基因专项抗旱玉米培育研究任务,通过促进根系发育创制20多份抗旱玉米材料,申报中间试验10多项,申报国家专利10多项和发表文章10多篇。

人员构成

吴忠义张中保张春王作平, 郑登俞李向龙孙晓荣

成果展示


[1]花粉管导入的玉米骨干自交系基因组编辑技术研究与应用(吴忠义主持北京市科技计划项目2017-2019,210万元)

[2]基因组编辑技术在玉米林草花的研究应用(参加院创新能力建设2018-2019,50万元)

[3]基于组学的玉米维管束形成机理解析(张中保主持院创新能力建设2017-2019,84万元

[4]玉米ZmFBL2互作蛋白的鉴定及其在抗旱调控中的作用(张中保主持市基金项目2017-2019,20万元)

[5]CKX3调控玉米根系发育的分子机理研究(张春主持院青年基金项目2017-2019,20万元)

[6]基于Als基因多位点编辑技术开发玉米除草剂筛选标记(王作平主持北京博士后项目2018-2019,7.3万元)

[7]重要农作物转基因新技术研究子课题(李向龙子课题主持院创新能力建设2017-2019,90万元)

[8]重要作物分子育种平台关键技术研发(张中保主持院创新能力建设2016-2018,75万元)

[9]促进根系发育来培育和胁迫响应的多价转基因抗旱玉米新品种培育(国家转基因专项重点课题2012ZX08003-003B, 2014-2016, 90万元,子课题主持)

[10]作物分子育种关键技术与新种质创制(参加院创新能力专项子课题105万元. 2014-2016)

[11]通过规模化转化鉴定玉米TIFY家族基因的抗逆功能(张中保主持市科技新星计划项目30万元. 2012-2015)
 

科研成果(论文、专利、获奖等)
[1]Kai Feng, Zigen Cai, Tao Ding, Hai Yan, Xiaolu Liu*, Zhongbao Zhang*. Effects of potassium-solubilizing and photosynthetic bacteria on tolerance to salt stress in maize. Journal of Applied Microbiology, 2019, 126, 1530-1540.

[2]Ye Wang, Haiyang Zhang, Hai Yan, Chunhua Yin, Yang Liu, Qianqian Xu, Xiaolu Liu *, Zhongbao Zhang*. Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain. Toxins, 2018, 10 (497): 1-16.

[3]Zhongbao Zhang, Zhibin Liang, Longfei Yin, Qing.X. Li*, Zhongyi Wu*. Distribution of four bioactive flavonoids in maize tissues of five varieties and correlation with expression of the biosynthetic genes. Journal of Agricultural and Food Chemistry, 2018, 66 (40): 10431-10437.(封面论文)

[4]Zhongbao Zhang∗, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu, Zhongyi Wu*. The complete genome sequence of Bacillus halotolerans ZB201702 isolated from a drought- and salt-stressed rhizosphere soil. Microbial Pathogenesis, 2018, 123: 246–249.

[5]Sheng He, Kai Feng, Tao Ding, Kaihui Huang, Hai Yan, Xiaolu Liu∗, Zhongbao Zhang*. Complete genome sequence of Bacillus licheniformis BL-010. Microbial Pathogenesis, 2018, 118:199–201
.
[6]Zhongbao Zhang, Xianglong Li, Chun Zhang, Huawen Zou, Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize nuclear factor Y gene families. Biochemical and Biophysical Research Communications, 2016, 478:752-758.

[7]Zhongbao Zhang, Xianglong Li, Rong Yu, Meng Han, Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Molecular Genetics and Genomics, 2015, 290:1849-1858.

[8]Zhongbao Zhang, Xianglong Li, Meng Han, Zhongyi Wu*. Genome-wide analysis and functional identification of the annexin gene family in maize (Zea mays L.). Plant Omics Journal, 2015, 8(5): 420-428.

[9]Huawen Zou, Xiaohan Wang, Conglin Huang, Jinsong Chen, Xiuhai Zhang, Chang Luo, Rong Yu and Zhongyi Wu*. Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition. Plant Omics Journal. 2014, 7: 94-101.

[10]Zhongbao Zhang, Yajuan Chen, Dan Zhao, Ruifen Li, Hongzhi Wang, Jiewei Zhang, Jianhua Wei*. X1-homologous genes family as central components in biotic and abiotic stresses response in maize (Zea mays L.). Functional& Integrative Genomics, 2014, 14: 101-110.

[11]Zhongbao Zhang, Jiewei Zhang, Yajuan Chen, Ruifen Li, Hongzhi Wang, Liping Ding, Jianhua Wei*. Isolation, structural analysis, and expression characteristics of the maize (Zea mays L.) hexokinase gene family. Molecular Biology Reports, 2014, 41:6157–6166.

[12]Zhongbao Zhang, Jiewei Zhang, Yajuan Chen, Ruifen Li, Hongzhi Wang, Jianhua Wei*. Genome-wide analysis and identification of HAK potassium transporter gene family in maize (Zea mays L.). Molecular Biology Reports, 39: 8465-8473, 2012.

[13] Xiaohan Wang, Binlin Liu, Conglin Huang, Xiuhai Zhang, Chang Luo, Xi Cheng, Rong Yu and Zhongyi Wu*. Over expression of Zmda1-1 gene increases seed mass of corn. African Journal of Biotechnology. 2012, 11(69): 13387-13395

[14]Jinsong Chen, Conglin Huang, Xiuhai Zhang, Rong Yu and Zhongyi Wu*. Detection of herbicide-resistant maize by using Loop-Mediated Isothermal Amplification of the pat selectable marker gene.  African Journal of Biotechnology, 2011, 10(75): 17055-17061.

[15]殷龙飞,王朝阳,吴忠义,张中保*,于荣*. 玉米ZmGRAS31 基因的克隆及功能研究. 作物学报,2019, 16.

[16]王犇,张春,李向龙,张中保,邹华文,吴忠义。利用微滴式数字PCR技术分析转基因玉米抗除草剂标记基因EPSP拷贝数,华北农学报,2017,32(3):70-76

[17]张中保,吴忠义,魏建华*. 玉米BURP家族基因的鉴定和分析. 玉米科学,2014,22(3):36-42.

[18]张中保,吴忠义*,魏建华*. 玉米1,3,4-三磷酸肌醇5-6 激酶ITPK家族基因的鉴定和分析. 科技导报,2014,33(16):46-50.

[19]吴忠义,黄丛林,张秀海等,发明授权专利名称“一种玉米ZmPti1基因启动子及其应用”,中国,2011,专利证书号:ZL200810227596.3。

[20]吴忠义,黄丛林,张秀海等,发明授权专利名称“一种利用LAMP法快速检测转基因玉米的方法”,中国,2012,专利证书号:ZL2010120766.5。

[21]吴忠义,黄丛林,张秀海等,发明授权专利名称“一种拟南芥根特异性表达基因ARSK1启动子的克隆方法”,中国,2013,专利证书号:ZL 201110359401.2

[22]张中保,吴忠义,李向龙,实用新型专利,授权2015年:ZL201520206083.X,一种玉米种子发芽瓶.

[23]张中保,吴忠义,李向龙,张春。实用新型专利,授权2016年:ZL201620424913.X,一种植物种子发芽可视化研究装置.

[24]张中保,吴忠义,李向龙,张春。实用新型专利,授权2016年:ZL201620424922.X,一种植物根系生长研究装置.

[25]李向龙,吴忠义,张晓东等,发明授权专利名称“一种提高玉米授粉效率的授粉方法”,中国,2016,专利号:ZL201510416431.0.

[26] 张春,吴忠义,李向龙,张中保,张国栋,王作平。实用新型专利授权“一种玉米水培及根系表型观测系统”,2019,专利号:ZL201821372435.9.