吴忠义
吴忠义博士，研究员。1969年生于福建德化，1988年7月至1998年6月就读于中国农业大学并获得学士、硕士和博士学位。2002年入选北京市科技新星；首都师范大学硕士生导师；北京市农林科学院博士后工作站博士后合作导师。 1998年7月至今在北京市农林科学院生物技术研究所工作。 1999年3月至2002年3月在美国马里兰大学细胞与分子遗传系作博士后。
研究方向
长期从事植物逆境分子生物学和生物技术育种方面工作，目前主要从事玉米分子育种工作，致力于建立和完善不依赖于基因型的玉米骨干自交系的高效DNA导入技术，建立和完善玉米骨干自交系的基因编辑技术体系，通过促进玉米根系生长培育抗旱玉米新种质材料。 熟悉学科领域：水分与矿质元素代谢与运输，作物抗逆生理，基因组编辑，植物激素与生长调节物质，作物分子育种，逆境信号转导等。
科研成果
近期主要主持参加项目
| [1] | 基于纳米磁珠介导的花粉转染法开发新型高效玉米转化体系（主持院创新能力储备性项目2019-2022，90万元） |
| [2] | 花粉管导入的玉米骨干自交系基因组编辑技术研究与应用（主持北京市科技计划项目2017-2020，210万元） |
| [3] | 基因组编辑技术在玉米林草花的研究应用（参加院创新能力建设2018-2019，50万元） |
| [4] | 基于组学的玉米维管束形成机理解析（参加院创新能力建设2017-2019，84万元） |
| [5] | 重要农作物转基因新技术研究子课题（参加院创新能力建设2017-2019，90万元） |
| [6] | 重要作物分子育种平台关键技术研发（参加院创新能力建设2016-2018，75万元） |
| [7] | 促进根系发育来培育和胁迫响应的多价转基因抗旱玉米新品种培育(国家转基因专项重点课题2012ZX08003-003B, 2014-2016, 90万元，子课题主持) |
| [8] | 作物分子育种关键技术与新种质创制（参加院创新能力专项子课题105万元. 2014-2016） |
主要代表论文及授权专利
| [1] | Chun Zhang, Ruijia Yang, Dengyu Zhen, Xianglong Li, Zhongbao Zhang*, Legong Li* and Zhongyi Wu*. ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays. Plant Growth Regulation, 2023 https://doi.org/10.1007/s10725-022-00946-2 |
| [2] | Zuoping Wang, Zhongbao Zhang, Dengyu Zheng, Tongtong Zhang, Xianglong Li, Chun Zhang, Rong Yu, Jianhua Wei* and Zhongyi Wu*. Efficient and genotype independent maize transformation using pollen transfected by DNA coated magnetic nanoparticles. J. Integr. Plant Biol. 2022, 64: 1145–1156. https://doi.org/10.1111/jipb.13263 |
| [3] | Zhongbao Zhang, Xiaoqin Tan, Xiaorong Sun, Jianhua Wei*, Qing Xiao Li* and Zhongyi Wu*. Isoorientin affects markers of Alzheimer’s disease via effects on the oral and gut microbiota in APP/PS1 mice. Journal of Nutrition, 2022, 152：140-152. |
| [4] | Chun Zhang, Xianglong Li, Zuoping Wang, Zhongbao Zhang* and Zhongyi Wu*. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157–5169. |
| [5] | Zhongbao Zhang, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu and Zhongyi Wu*. Analyses of the Complete Genome Sequence of the Strain Bacillus pumilus ZB201701 Isolated from Rhizosphere Soil of Maize under Drought and Salt Stress. Microbes Environ.2019,34(3): 310-315. |
| [6] | Zhongbao Zhang, Zhibin Liang, Longfei Yin, Qingxiao Li* and Zhongyi Wu*. Distribution of four bioactive flavonoids in maize tissues of five varieties and correlation with expression of the biosynthetic genes. Journal of Agricultural and Food Chemistry, 2018, 66 (40): 10431-10437. （封面论文） |
| [7] | Zhongbao Zhang, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu and Zhongyi Wu*. The complete genome sequence of Bacillus halotoleransZB201702 isolated from a drought- and salt-stressed rhizosphere soil. Microbial Pathogenesis, 2018, 123: 246–249. |
| [8] | Zhongbao Zhang, Xianglong Li, Chun Zhang and Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize nuclear factor Y gene families. Biochemical and Biophysical Research Communications. 2016, 478: 752-758. |
| [9] | Zhongbao Zhang, Xianglong Li, Rong Yu, Meng Han and Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Mol Genet Genomics. 2015, 290: 1845-1858. |
| [10] | Huawen Zou, Xiaohan Wang, Conglin Huang, Jinsong Chen, Xiuhai Zhang, Chang Luo, Rong Yu and Zhongyi Wu*. Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition. Plant Omics Journal. 2014, 7: 94-101. |
| [11] | Xiaohan Wang, Binlin Liu, Conglin Huang, Xiuhai Zhang, Chang Luo, Xi Cheng, Rong Yu and Zhongyi Wu*. Over expression of Zmda1-1 gene increases seed mass of corn. African Journal of Biotechnology. 2012, 11(69): 13387-13395 |
| [12] | Jinsong Chen, Conglin Huang, Xiuhai Zhang, Rong Yu and Zhongyi Wu*. Detection of herbicide-resistant maize by using Loop-Mediated Isothermal Amplification of the pat selectable marker gene. African Journal of Biotechnology, 2011, 10(75): 17055-17061 |
| [13] | Yongmei Zhang, Zhongyi Wu and Rong Yu. Rearrangements of microtubule cytoskeleton in stomatal closure of Arabidopsis induced by nitric oxide. 2008, Chinese Science Bulletin, 2008, 53(6):848-852. |
| [14] | Huawen Zou, Zhongyi Wu, XiuhaiZhang, Qin Yang, Wensuo Jia and Conglin Huang. Gene expression analysis of ZmPti1, encoding a maize Pti like kinase, suggest a role in stress signalling. Plant Science, 2006, 171: 99-105 |
| [15] | Yuhua Wang, Zhongyi Wu, Xiuhai Zhang, Guoqiang Chen, Qiong Wu, Conglin Huang and Qing Yang. Synthesis of medium-chain-length-polyhydroxyalkanoates in tobacco via chloroplast genetic engineering. Chinese Science Bulletin, 2005, 50: 1113-1120 |
| [16] | Zhongyi Wu, Feng Liang, Bimei Hong, Jeff C. Young, Michael R. Sussman, Jeffrey F. Harper and Heven Sze. An ER-bound Ca2+/Mn2+pump, ECA1, supports plant growth and confers tolerance to Mn2+stress. Plant Physiol. 2002, 130：128-137. |
| [17] | 吴忠义，黄丛林，张秀海等，授权专利名称“一种玉米ZmPti1基因启动子及其应用”，中国，2011，专利证书号：ZL200810227596.3。 |
| [18] | 吴忠义，黄丛林，张秀海等，授权专利名称“一种利用LAMP法快速检测转基因玉米的方法”，中国，2012，专利证书号：ZL2010120766.5。 |
| [19] | 吴忠义，黄丛林，张秀海等，授权专利名称“一种拟南芥根特异性表达基因ARSK1启动子的克隆方法”，中国，2013，专利证书号：ZL 201110359401.2 |
| [20] | 李向龙，吴忠义，张晓东等，授权专利名称“一种提高玉米授粉效率的授粉方法”，中国，2016，专利号：ZL201510416431.09401.2 |
| [21] | 王作平，吴忠义，张中保，李向龙，张春。授权专利名称“一种不依赖于玉米基因型的DNA导入方法” 专利号：ZL201910623296.5 |
| [22] | 张中保，吴忠义，郑登俞，王作平，张春，李向龙。授权专利名称“一种改良的玉米花粉磁转染方法”专利号：ZL202111418727.8 |
| [23] | 吴忠义，张中保，王作平，郑登俞，张春，李向龙。授权专利名称“一种制备转基因玉米花粉的方法及其使用的试剂盒” 专利号：ZL202210532986.1 |
