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科研队伍
研究室
玉米分子育种研究室

研究室介绍

玉米分子育种研究室研究方向主要集中在以下三个方面:建立和完善不依赖于基因型的玉米骨干自交系的高效DNA导入技术,建立和完善玉米骨干自交系的基因编辑技术体系,通过促进玉米根系生长培育抗旱玉米新种质材料。二是聚焦营养导向型农业,以玉米天然产物(类黄酮等)合成通路解析为抓手,挖掘通路关键基因,解析基因调控机制,创制品质优良的功能型玉米新品种;三是玉米根系生长发育和抗逆性相关重要基因的挖掘及功能鉴定和基于RNAi的新型绿色高效玉米害虫防治技术研究。近年来在玉米天然产物合成通路解析、类黄酮异荭草素营养保健功能、玉米基因组编辑、玉米高效遗传转化方法、玉米根系发育等方面取得较大进展;获得1项重大技术突破:建立了具有我国自主知识产权的“纳米磁珠介导的不依赖于基因型的玉米高效转化平台”。

人员构成

吴忠义张中保张春郑登俞柴一萍

成果展示

[1]北京市农林科学院创新能力建设专项(主持):“重要农作物转基因新种质创制”(2023.01- 2025.12);
[2]北京市自然科学基金面上项目(主持):“玉米ZmJAZ12互作蛋白的鉴定及其在抗旱调控通路中的作用”(2022.1-2024.12);
[3]北京市农林科学院创新能力建设专项(主持):“基于RNAi的新型玉米害虫防治技术研究”(2022.01-2024.12);
[4]国家自然科学基金青年基金(主持):“ZmbHLH161调控玉米根系生长发育的分子机理研究”(2021.01-2023.12);
[5]北京市农林科学院(基金培育专项)(主持):“玉米黄酮异荭草素合成通路关键基因ZmCGT1功能解析”(2021.01- 2021.12);
[6]北京市科学技术协会(金桥工程资金)(主持),“玉米黄酮类物质异荭草苷缓解阿尔茨海默病的机理研究及相关功能食品研发”(2021.06-2023.06)
[7]北京市农林科学院创新能力建设专项(主持):“基于纳米磁珠介导的花粉转染法开发新型高效玉米转化体系”(2019-2022)
[8]北京市科技计划项目(主持):“花粉管导入的玉米骨干自交系基因组编辑技术研究与应用”(2017-2020)
[9]北京市自然科学基金面上项目(主持):“玉米ZmFBL2互作蛋白的鉴定及其在抗旱调控中的作用”(2017.1-2019.12);
[10]国家转基因专项重点课题(子课题主持):“促进根系发育来培育和胁迫响应的多价转基因抗旱玉米新品种培育”(2012ZX08003-003B, 2014-2016)
[11]北京市农林科学院创新能力专项(主持):“基于组学的玉米维管束形成机理解析”(2017.1-2019.12);
[12]北京市农林科学院生物技术共享平台(主持):“重要作物分子育种平台关键技术研发”(2016-2018);
[13]北京市科技新星计划项目(主持):“通过规模化转化鉴定玉米TIFY家族基因的抗逆功能”(2012.12-2015.12);
[14]北京市农林科学院青年基金(主持):“CKX3调控玉米根系生长发育的分子机理研究” (2017.01-2019.12);


科研成果:


论文、专利、获奖等

[1]Zuoping Wang#, Zhongbao Zhang#, Dengyu Zheng, Tongtong Zhang, Xianglong Li, Chun Zhang, Rong Yu,Jianhua Wei, Zhongyi Wu. Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticles. Journal of Integrative Plant Biology, 2022, 64: 1145–1156.
[2]Xiaorong Sun, Xiaofeng Xue, Xiaqing Wang, Chun Zhang, Dengyu Zheng, Wei Song, Jiuran Zhao, Jianhua Wei, Zhongyi Wu, Zhongbao Zhang*. Natural variation of ZmCGT1 is responsible for the isoorientin accumulation in maize silk. Plant Journal, 2022, 109: 64–76.
[3]Zhongbao Zhang#, Xiaoqin Tan#, Xiaorong Sun, Jianhua Wei*, Qing X. Li*, Zhongyi Wu*. Isoorientin affects markers of Alzheimer's disease via effects on the oral and gut microbiota in APP/PS1 mice. Journal of Nutrition, 2022, 152: 140–152.
[4]Tongtong Zhang, Dengyu Zheng, Chun Zhang, Zhongyi Wu, Rong Yu*, Zhongbao Zhang*. Heterologous expression of ZmNF‑YA12 confers tolerance to drought and salt stress in Arabidopsis. Plant Biotechnology Reports (2022) 16:437–448.
[5]Chun Zhang, Xianglong Li, Zuoping Wang, Zhongbao Zhang*, Zhongyi Wu*. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157–5169.
[6]Chun Zhang, Ruijia Yang, Tongtong Zhang, Dengyu Zheng, Xianglong Li, Zhongbao Zhang*, Legong Li*, Zhongyi Wu*. ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays. Plant Growth Regulation, 2023. https://doi.org/10.1007/s10725-022-00946-2
[7]Chun Zhang, Xianglong Li, Longfei Yin, Cai Liu, Huawen Zou, Zhongbao Zhang∗, Zhongyi Wu∗. Analysis of the complete genome sequence of Brevibacterium frigoritolerans ZB201705 isolated from drought- and salt-stressed rhizosphere soil of maize. Annals of Microbiology, 2019, 69: 1489–1496.
[8]Zhongbao Zhang∗, Longfei Yin, Xianglong Li, Chun Zhang, Huawen Zou, Cai Liu, Zhongyi Wu*. Analyses of the Complete Genome Sequence of the Strain Bacillus pumilus ZB201701 Isolated from Rhizosphere Soil of Maize under Drought and Salt Stress. Microbes and Environments, 2019, 34:310-315.
[9]Zigen Cai, Kai Feng, Xin Li, Hai Yan, Zhongbao Zhang*, Xiaolu Liu. Pre‑breeding: the role of antioxidant enzymes on maize in salt stress tolerance. Acta Physiologiae Plantarum, 2019, 41:102. (Q2,WOS:000468289800004)
[10]Kai Feng, Zigen Cai, Tao Ding, Hai Yan, Xiaolu Liu*, Zhongbao Zhang*. Effects of potassium-solubilizing and photosynthetic bacteria on tolerance to salt stress in maize. Journal of Applied Microbiology, 2019, 126, 1530-1540.
[11]Ye Wang, Haiyang Zhang, Hai Yan, Chunhua Yin, Yang Liu, Qianqian Xu, Xiaolu Liu *, Zhongbao Zhang*. Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain. Toxins, 2018, 10 (497): 1-16.
[12]Zhongbao Zhang, Zhibin Liang, Longfei Yin, Qing.X. Li*, Zhongyi Wu*. Distribution of four bioactive flavonoids in maize tissues of five varieties and correlation with expression of the biosynthetic genes. Journal of Agricultural and Food Chemistry, 2018, 66 (40): 10431-10437.(封面论文)
[13]Zhongbao Zhang∗, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu, Zhongyi Wu*. The complete genome sequence of Bacillus halotolerans ZB201702 isolated from a drought- and salt-stressed rhizosphere soil. Microbial Pathogenesis, 2018, 123: 246–249.
[14]Sheng He, Kai Feng, Tao Ding, Kaihui Huang, Hai Yan, Xiaolu Liu∗, Zhongbao Zhang∗. Complete genome sequence of Bacillus licheniformis BL-010. Microbial Pathogenesis, 2018, 118:199–201.
[15]Zhongbao Zhang, Xianglong Li, Chun Zhang, Huawen Zou, Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize nuclear factor Y gene families. Biochemical and Biophysical Research Communications, 2016, 478:752-758.
[16]Zhongbao Zhang, Xianglong Li, Rong Yu, Meng Han, Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Molecular Genetics and Genomics, 2015, 290:1849-1858.
[17]Zhongbao Zhang, Yajuan Chen, Dan Zhao, Ruifen Li, Hongzhi Wang, Jiewei Zhang, Jianhua Wei*. X1-homologous genes family as central components in biotic and abiotic stresses response in maize (Zea mays L.). Functional& Integrative Genomics, 2014, 14: 101-110.
[18]Zhongbao Zhang, Jiewei Zhang, Yajuan Chen, Ruifen Li, Hongzhi Wang, Liping Ding, Jianhua Wei*. Isolation, structural analysis, and expression characteristics of the maize (Zea mays L.) hexokinase gene family. Molecular Biology Reports, 2014, 41:6157–6166.
[19]Zhongbao Zhang, Jiewei Zhang, Yajuan Chen, Ruifen Li, Hongzhi Wang, Jianhua Wei*. Genome-wide analysis and identification of HAK potassium transporter gene family in maize (Zea mays L.). Molecular Biology Reports, 39: 8465-8473, 2012.
[20]Zhongbao Zhang, Huiyong Li, Dengfeng Zhang, Yinghui Liu, Jing Fu, Yunsu Shi, Yanchun Song, Tianyu Wang*, Yu Li*. Characterization and expression analysis of six MADS-box genes in maize (Zea mays L.). Journal of Plant Physiology, 169: 797-806, 2012.
[21]Jiewei Zhang, Zhongbao Zhang, Dan Zhu, Yang Guan, Dianyi Shi, Yajuan Chen, Ruifen Li, Hongzhi Wang, Jianhua Wei*. Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa. Journal of Plant Biochemistry and Biotechnology, 2015, 24: 338-346.
[22]Zhongbao Zhang, Xianglong Li, Meng Han, Zhongyi Wu*. Genome-wide analysis and functional identification of the annexin gene family in maize (Zea mays L.). Plant Omics Journal, 2015, 8(5): 420-428.
[23]张彤彤,郑登俞,吴忠义,张中保*,于荣*. 玉米NF-Y转录因子基因 ZmNF-YB13响应干旱和盐胁迫的功能分析. 生物技术通报,2022: 38(10).
[24]杨锐佳,张中保*,吴忠义*. 植物转录因子TIFY家族蛋白结构和功能的研究进展. 生物技术通报,2020,36 (12): 121−128.
[25]殷龙飞,王朝阳,吴忠义,张中保*,于荣*. 玉米ZmGRAS31 基因的克隆及功能研究. 作物学报,2019, 16.
[26]悦曼芳, 张春*, 吴忠义*. 植物转录因子AP2/ERF家族蛋白结构和功能的研究进展[J]. 生物技术通报, 2022, 38(12): 11-26.
[27]悦曼芳#, 张春#, 郑登俞, 邹华文*, 吴忠义*. 玉米转录因子ZmbHLH91对非生物逆境胁迫的应答[J]. 作物学报, 2022, 48(12): 3004-3017.
[28]杨梦婷#,张春#,王作平,邹华文*,吴忠义*. 玉米ZmbHLH161基因的克隆及功能研究[J]. 作物学报, 2020, 46(11): 1798-1806.
[29]王犇#,张春#,李向龙,张中保,邹华文*,吴忠义*. 利用微滴式数字PCR技术分析转基因玉米抗除草剂标记基因EPSP拷贝数[J]. 华北农学报, 2017, 32(3): 70-76.
[30]张中保,吴忠义,魏建华*. 玉米BURP家族基因的鉴定和分析. 玉米科学,2014,22(3):36-42.
[31]张中保,吴忠义*,魏建华*. 玉米1,3,4-三磷酸肌醇5-6 激酶ITPK家族基因的鉴定和分析. 科技导报,33(16):46-50.
[32]发明专利,授权2021,ZL201910623296.5,一种不依赖于玉米基因型的DNA导入方法,王作平、吴忠义、张中保、李向龙、张春。
[33]发明专利,授权2022,ZL202210532986.1,一种制备转基因玉米花粉的方法及其使用的试剂盒,吴忠义、张中保、王作平、郑登俞、张春、李向龙。
[34]发明专利,授权2022,ZL202111418727.8,一种改良的玉米花粉磁转染方法,张中保、吴忠义、郑登俞、王作平、张春、李向龙。
[35]实用新型专利,授权2015年:ZL201520206083.X,一种玉米种子发芽瓶. 张中保,吴忠义,李向龙。
[36]实用新型专利,授权2021年:ZL202120562216.2,一种作物幼苗根系表型观察及鉴定装置. 张春,吴忠义,张中保,李向龙,郑登俞,悦曼芳。
[37]实用新型专利,授权2020年:ZL201921540728.8,玉米苗期水培及根系表型观察系统. 张春,吴忠义,李向龙,张中保,王作平,郑登俞。
[38]实用新型专利,授权2019年:ZL201821372457.5,一种用于连通玉米水培装置的输液管道及玉米水培装置组件. 张春,吴忠义,李向龙,张中保,张国栋,王作平。
[39]实用新型专利,授权2019年:ZL201821373054.2,一种用于玉米水培和根系表型观察的圆通装置. 张春,吴忠义,李向龙,张中保,张国栋,王作平。
[40]实用新型专利,授权2016年:ZL201620424913.X,一种植物种子发芽可视化研究装置. 张中保,吴忠义,李向龙,张春。
[41]实用新型专利,授权2016年:ZL201620424922.X,一种植物根系生长研究装置. 张中保,吴忠义,李向龙,张春。
[42]实用新型专利,授权2017年:ZL201620470711.X,一种提高玉米花粉管通道转化效率的装置. 李向龙,吴忠义,张中保,张春,张国栋,赵青叶。
[43]实用新型专利,授权2017年:ZL201720558074.6,一种玉米单穗脱粒机. 李向龙,吴忠义,张中保,张春,张国栋,赵青叶。
[44]实用新型专利,授权2018年:ZL201820036147.X,一种抽吸玉米雌穗汁液的装置. 李向龙,吴忠义,张中保,张春,张国栋,赵青叶。